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Amino Acid Frequency

Region Length

Numbering Scheme Chothia Martin Kabat AHo IMGT Chain Type Heavy Light Organism All Acanthopagrus schlegelii Acipenser Acipenser baeri Acipenser baerii Acipenser ruthenus Acipenser schrenckii Aequorea victoria, vicugna pacos Amblyraja georgiana Amblyraja hyperborea Ambystoma mexicanum Amia calva Amphibia anura Amphiprion clarkii Anarhichas minor Anas Anas platyrhynchos Anas platyrhynchos domestica Anas poecilorhyncha Andrias davidianus Anguilla anguilla Anguilla japonica Anolis carolinensis Anoplopoma fimbria Anser Anser anser Anser anser domesticus Anser cygnoides Anser cygnoides orientalis Anser sp. gighv2011 Anser sp. giglv2009 Aotus Aotus nancymaae Apis cerana Atherina boyeri Bacillus thuringiensis serovar kyushu... Bathyraja albomaculata Bathyraja brachyurops Bathyraja eatonii Bos Bos taurus Bovichtus diacanthus Bubalus bubalis Caiman Caiman crocodilus Cairina moschata Callithrix jacchus Callorhinchus milii Camelidae Camelidae mixed library Camelus Camelus bactrianus Camelus dromedarius Canis Canis lupus familiaris Capra hircus Carassius Carassius auratus Carassius gibelio Carchachinus plumbeus Carcharhinus Carcharhinus leucas Carcharhinus plumbeus Carcharinus Cavia porcellus Cetacea Chaenocephalus aceratus Channa argus Chionodraco hamatus Chlorocebus sabaeus Chrysolophus pictus Cloning vector pask88-huhrs3-vh-ep3/1 Cloning vector pcag-scfvgcn4sfgfptet1cd Cloning vector pcomb3h Cloning vector pdsg253 Cloning vector pdsg289 Cloning vector pdsg290 Cloning vector pdsg312 Cloning vector pdsg320 Cloning vector pdsg321 Cloning vector pdsg339 Cloning vector pdsg361 Cloning vector pdsg362 Cloning vector pdsg363 Cloning vector pdsg364 Cloning vector pdsg365 Cloning vector pdsg366 Cloning vector pdsg367 Cloning vector pdsg368 Cloning vector pdsg369 Cloning vector pdsg370 Cloning vector pdsg371 Cloning vector pdsg372 Cloning vector pdsg373 Cloning vector pdsg374 Cloning vector pdsg375 Cloning vector pdsg376 Cloning vector pdsg377 Cloning vector pdsg378 Cloning vector pdsg379 Cloning vector pdsg380 Cloning vector pdsg381 Cloning vector pdsg382 Cloning vector pdsg383 Cloning vector pdsg384 Cloning vector pdsg385 Cloning vector pdsg386 Cloning vector pdsg387 Cloning vector pdsg388 Cloning vector pdsg389 Cloning vector pdsg390 Cloning vector pdsg391 Cloning vector pdsg392 Cloning vector pdsg393 Cloning vector pdsg394 Cloning vector pdsg395 Cloning vector pdsg396 Cloning vector pdsg397 Cloning vector pdsg398 Cloning vector pdsg399 Cloning vector pdsg400 Cloning vector pdsg401 Cloning vector pdsg402 Cloning vector pdsg403 Cloning vector pdsg404 Cloning vector pdsg405 Cloning vector pdsg406 Cloning vector pdsg407 Cloning vector pdsg408 Cloning vector pdsg409 Cloning vector pdsg410 Cloning vector pdsg411 Cloning vector pdsg412 Cloning vector pmab136 Cloning vector pnb102 Cloning vector pplattet-grna2 Cloning vector psb4k5-cadc-vhh-caffeine Cloning vector psb4k5-lexa-vhh-caffeine Cloning vector pzgt5 Columba livia Coturnix japonica Cricetinae Cricetinae gen. sp. Cricetulus migratorius Ctenopharyngodon idella Cynops pyrrhogaster Cyprinus carpio Danio rerio Dasypus novemcinctus Devario aequipinnatus Dicentrarchus labrax Dipnoi Drosophila melanogaster Elops Elops saurus Epinephelus akaara Epinephelus coioides Eptatretus burgeri Equus caballus Escherichia coli Escherichia coli k-12 Esox lucius Eudontomyzon morii Expression vector pste2-215 Expression vector pvt1 Expression vector pvt2 Expression vector pvt3 Expression vector pvt4 Felis catus Gadus macrocephalus Gadus morhua Gallus Gallus domesticus Gallus gallus Geomvidae Ginglymostoma cirratum Gorilla gorilla Gymnodraco acuticeps Hemibagrus macropterus Heterodontus francisci Hippoglossus hippoglossus Homo Homo sapiens Homo sapiens, mus musculus Homo sapiens, rattus norvegicus Homo sapiens, synthetic construct Homo sapiens/mus musculus xenograft Human cytomegalovirus, lama glama Human orfeome gateway entry vector Hybrid Hydrolagus Hydrolagus colliei Ictalurus furcatus Ictalurus punctatus Isoodon macrourus Labeo rohita Labrus bergylta Lama Lama glama Lama peruviana Larimichthys crocea Lateolabrax japonicus Latimeria menadoensis Latris lineata Lepisosteidae Lepisosteus osseus Leporidae Lepus Lepus europaeus Lepus granatensis Leucoraja erinacea Lissamphibia Liza aurata Llama glama Lutjanus sanguineus Lyrurus tetrix Macaca Macaca fascicularis Macaca mulatta Macropodidae Mammalian expression vector pcaggse6l Mammalian expression vector pef-bosbs... Mammalian expression vector pexpr156 Mammalian expression vector pexpr166 Mammalian expression vector pexpr21 Mammalian expression vector pexpr37 Mammalian expression vector pexpr38 Mammalian expression vector pexpr39 Mammalian expression vector pexpr5 Mammalian expression vector pexpr50 Mammalian expression vector pexpr51 Mammalian expression vector pexpr53 Mammalian expression vector pexpr61 Mammalian expression vector pexpr62 Mammalian expression vector pexpr63 Mammalian expression vector pexpr64 Mammalian expression vector pexpr65 Mammalian expression vector pexpr66 Mammalian expression vector pexpr71 Mammalian expression vector pexpr72 Mammalian expression vector psv23se6h Mammalian expression vector psv23se6hf1 Mammalian expression vector psv23se6hm Mammalian expression vector psv23se6hmde Mammalian expression vector psv23se6hme Mammalian expression vector psv23se6hmk Mammalian expression vector psv23se6hmrk Mammalian expression vector psv51b1sfv Mammalian expression vector psv51e6h Mammalian expression vector psv51e6hf1 Mammalian expression vector psv51e6hf2 Mammalian expression vector psv51e6hmde Mammalian expression vector psv51e6hme Mammalian expression vector psv51e6hmk Mammalian expression vector psv51e6hmrk Marmota monax Megalobrama amblycephala Melanogrammus aeglefinus Meleagris gallopavo Miichthys miiuy Monodelphis domestica Murinae gen. sp. Murine adenovirus 1 Mus Mus musculoides Mus musculus Mus musculus albula Mus musculus castaneus Mus musculus domesticus Mus musculus molossinus Mus musculus musculus Mus musculus, homo sapiens Mus musculus, synthetic construct Mus musculus,homo sapiens Mus pahari Mus sp. Mus spretus Mustela Mustela sp. Myotis lucifugus Myxocyprinus asiaticus Neovison vison Nomascus leucogenys, homo sapiens Notamacropus eugenii Notothenia coriiceps Nyctereutes procyonoides Nycticebus coucang Oncorhynchus Oncorhynchus mykiss Onychogalea fraenata Orectolobus maculatus Orectolobus maculatus, homo sapiens Oreochromis niloticus Ornithorhynchus anatinus Oryctolagus Oryctolagus cuniculus Oryctolagus cuniculus algirus Oryctolagus cuniculus, homo sapiens Oryzias latipes Oves Ovis aries Pagrus major Pan Pan troglodytes Papio anubis Paralichthys olivaceus Pelodiscus sinensis Phagemid vector pcomb3x-kd247scfv-vi Phagemid vector pgald7 Phagemid vector pgald7dl Phagemid vector pgald9 Phagemid vector pgald9dl Phasianidae meleagris Pinnipedia Pisces Plasmid pad1 Plecoglossus altivelis Plecoglossus altivelis altivelis Pleurodeles waltl Polyodontidae Protopterus aethiopicus Protopterus annectens Protopterus dolloi Pseudemys scripta elegans Pseudobatos productus Pteropus alecto Rachycentron canadum Raja eglanteria Raja erinacea Rajiformes dasyatidae Rana catesbeiana Rattus Rattus norvegicus Rattus rattus Rattus sp. Rattus villosissimus Rattus, homo sapiens Red display vector Rpl23-fusion expression vector pscfv Salamandridae Salmo salar Salvelinus alpinus Sarcophilus harrisii Schizothorax prenanti Scophthalmus maximus Scyliorhinus canicula Seriola quinqueradiata Serpentes Silurus meridionalis Siniperca chuatsi Sparus aurata Sphoeroides nephelus Squaliobarbus curriculus Squalus acanthias Stegastes partitus Streptomyces avidinii Struthio camelus Sus Sus scrofa Sylvilagus floridanus Synthetic construct Synthetic plasmid prk122 Synthetic plasmid prk123 Synthetic plasmid puc57-hdr-hel23 Systomus sarana Tachyglossus aculeatus Tachysurus fulvidraco Taeniopygia guttata Takifugu rubripes Testudines Tetraodon nigroviridis Thunnus orientalis Tokudaia muenninki Trachemys scripta elegans Trematomus bernacchii Triakis scyllium Trichechus manatus latirostris Trichosurus vulpecula Tursiops truncatus Tympanuchus cupido Unclassified Unclassified sequences Unidentified Unidentified cloning vector Unknown Vector patce6hy3 Vector pbre6l Vector pdmiphoae6l Vector pqe2c11sfv Vector pqeb1sfv Vector tpmv_p-egfp_hacd20 Vector tpmv_p-egfp_haegfr Vicugna pacos Xenopus Xenopus laevis Xenopus laevis/gilli Xenopus leavis Xenopus tropicalis Yeast expression vector pscudmfe6l2 Yeast expression vector pscudt2mfe6h Yeast expression vector pscudt2mfe6l Position H1 H2 H3 H4 H5 H6 H6A H6B H6C H6D H7 H8 H9 H10 H11 H12 H13 H14 H15 H16 H17 H18 H19 H20 H21 H22 H23 H24 H25 H26 H27 H28 H29 H30 H31 H31A H31B H31C H31D H31E H31F H31G H31H H32 H33 H34 H35 H36 H37 H38 H39 H40 H41 H42 H43 H44 H45 H46 H47 H48 H49 H50 H51 H52 H52A H52B H52C H52D H52E H52F H52G H53 H54 H55 H56 H57 H58 H59 H60 H61 H62 H63 H64 H65 H66 H67 H68 H69 H70 H71 H72 H73 H74 H75 H76 H77 H78 H79 H80 H81 H82 H82A H82B H82C H82D H82E H83 H84 H85 H86 H87 H88 H89 H90 H91 H92 H93 H94 H95 H96 H97 H98 H99 H100 H100A H100B H100C H100D H100E H100F H100G H100H H100I H100J H100K H100L H100M H100N H100O H100P H100Q H100R H100S H100T H101 H102 H103 H104 H105 H106 H107 H108 H109 H110 H111 H112 H113 H114 Distribution for Chothia position H1 Amino Acid Non- identical Seqs Relative Frequency A 655 1% C 877 2% D 1416 3% E 20609 39% F 4 <1% G 977 2% H 99 <1% I 45 <1% K 136 <1% L 433 <1% Amino Acid Non- identical Seqs Relative Frequency M 21 <1% N 46 <1% P 121 <1% Q 26448 50% R 365 <1% S 359 <1% T 146 <1% V 149 <1% W 4 <1% Y 14 <1% - 124 <1% Total 53048 100%

Region Definition Chothia AbM Kabat Contact IMGT Chain Type / Class Heavy Heavy Alpha Heavy Alpha 1 Heavy Alpha 2 Heavy Gamma Heavy Gamma 1 Heavy Gamma 2 Heavy Gamma 2 A Heavy Gamma 2 B Heavy Gamma 2 C Heavy Gamma 3 Heavy Gamma 4 Heavy Delta Heavy Epsilon Heavy Xi Heavy Mu Heavy Nu Heavy Omega Heavy Phi Heavy Pi Heavy Rho Heavy Tau Heavy Upsilon Heavy Zeta Light Light Kappa Light Lambda Light Iota Light Iota 1 Light Iota 2 Light Iota 3 Light Iota 4 Light Rho Light Sigma Organism All Acanthopagrus schlegelii Acipenser Acipenser baeri Acipenser baerii Acipenser ruthenus Acipenser schrenckii Aequorea victoria, vicugna pacos Amblyraja georgiana Amblyraja hyperborea Ambystoma mexicanum Amia calva Amphibia anura Amphiprion clarkii Anarhichas minor Anas Anas platyrhynchos Anas platyrhynchos domestica Anas poecilorhyncha Andrias davidianus Anguilla anguilla Anguilla japonica Anolis carolinensis Anoplopoma fimbria Anser Anser anser Anser anser domesticus Anser cygnoides Anser cygnoides orientalis Anser sp. gighv2011 Anser sp. giglv2009 Aotus Aotus nancymaae Apis cerana Atherina boyeri Bacillus thuringiensis serovar kyushu... Bathyraja albomaculata Bathyraja brachyurops Bathyraja eatonii Bos Bos taurus Bovichtus diacanthus Bubalus bubalis Caiman Caiman crocodilus Cairina moschata Callithrix jacchus Callorhinchus milii Camelidae Camelidae mixed library Camelus Camelus bactrianus Camelus dromedarius Canis Canis lupus familiaris Capra hircus Carassius Carassius auratus Carassius gibelio Carchachinus plumbeus Carcharhinus Carcharhinus leucas Carcharhinus plumbeus Carcharinus Cavia porcellus Cetacea Chaenocephalus aceratus Channa argus Chionodraco hamatus Chlorocebus sabaeus Chrysolophus pictus Cloning vector pask88-huhrs3-vh-ep3/1 Cloning vector pcag-scfvgcn4sfgfptet1cd Cloning vector pcomb3h Cloning vector pdsg253 Cloning vector pdsg289 Cloning vector pdsg290 Cloning vector pdsg312 Cloning vector pdsg320 Cloning vector pdsg321 Cloning vector pdsg339 Cloning vector pdsg361 Cloning vector pdsg362 Cloning vector pdsg363 Cloning vector pdsg364 Cloning vector pdsg365 Cloning vector pdsg366 Cloning vector pdsg367 Cloning vector pdsg368 Cloning vector pdsg369 Cloning vector pdsg370 Cloning vector pdsg371 Cloning vector pdsg372 Cloning vector pdsg373 Cloning vector pdsg374 Cloning vector pdsg375 Cloning vector pdsg376 Cloning vector pdsg377 Cloning vector pdsg378 Cloning vector pdsg379 Cloning vector pdsg380 Cloning vector pdsg381 Cloning vector pdsg382 Cloning vector pdsg383 Cloning vector pdsg384 Cloning vector pdsg385 Cloning vector pdsg386 Cloning vector pdsg387 Cloning vector pdsg388 Cloning vector pdsg389 Cloning vector pdsg390 Cloning vector pdsg391 Cloning vector pdsg392 Cloning vector pdsg393 Cloning vector pdsg394 Cloning vector pdsg395 Cloning vector pdsg396 Cloning vector pdsg397 Cloning vector pdsg398 Cloning vector pdsg399 Cloning vector pdsg400 Cloning vector pdsg401 Cloning vector pdsg402 Cloning vector pdsg403 Cloning vector pdsg404 Cloning vector pdsg405 Cloning vector pdsg406 Cloning vector pdsg407 Cloning vector pdsg408 Cloning vector pdsg409 Cloning vector pdsg410 Cloning vector pdsg411 Cloning vector pdsg412 Cloning vector pmab136 Cloning vector pnb102 Cloning vector pplattet-grna2 Cloning vector psb4k5-cadc-vhh-caffeine Cloning vector psb4k5-lexa-vhh-caffeine Cloning vector pzgt5 Columba livia Coturnix japonica Cricetinae Cricetinae gen. sp. Cricetulus migratorius Ctenopharyngodon idella Cynops pyrrhogaster Cyprinus carpio Danio rerio Dasypus novemcinctus Devario aequipinnatus Dicentrarchus labrax Dipnoi Drosophila melanogaster Elops Elops saurus Epinephelus akaara Epinephelus coioides Eptatretus burgeri Equus caballus Escherichia coli Escherichia coli k-12 Esox lucius Eudontomyzon morii Expression vector pste2-215 Expression vector pvt1 Expression vector pvt2 Expression vector pvt3 Expression vector pvt4 Felis catus Gadus macrocephalus Gadus morhua Gallus Gallus domesticus Gallus gallus Geomvidae Ginglymostoma cirratum Gorilla gorilla Gymnodraco acuticeps Hemibagrus macropterus Heterodontus francisci Hippoglossus hippoglossus Homo Homo sapiens Homo sapiens, mus musculus Homo sapiens, rattus norvegicus Homo sapiens, synthetic construct Homo sapiens/mus musculus xenograft Human cytomegalovirus, lama glama Human orfeome gateway entry vector Hybrid Hydrolagus Hydrolagus colliei Ictalurus furcatus Ictalurus punctatus Isoodon macrourus Labeo rohita Labrus bergylta Lama Lama glama Lama peruviana Larimichthys crocea Lateolabrax japonicus Latimeria menadoensis Latris lineata Lepisosteidae Lepisosteus osseus Leporidae Lepus Lepus europaeus Lepus granatensis Leucoraja erinacea Lissamphibia Liza aurata Llama glama Lutjanus sanguineus Lyrurus tetrix Macaca Macaca fascicularis Macaca mulatta Macropodidae Mammalian expression vector pcaggse6l Mammalian expression vector pef-bosbs... Mammalian expression vector pexpr156 Mammalian expression vector pexpr166 Mammalian expression vector pexpr21 Mammalian expression vector pexpr37 Mammalian expression vector pexpr38 Mammalian expression vector pexpr39 Mammalian expression vector pexpr5 Mammalian expression vector pexpr50 Mammalian expression vector pexpr51 Mammalian expression vector pexpr53 Mammalian expression vector pexpr61 Mammalian expression vector pexpr62 Mammalian expression vector pexpr63 Mammalian expression vector pexpr64 Mammalian expression vector pexpr65 Mammalian expression vector pexpr66 Mammalian expression vector pexpr71 Mammalian expression vector pexpr72 Mammalian expression vector psv23se6h Mammalian expression vector psv23se6hf1 Mammalian expression vector psv23se6hm Mammalian expression vector psv23se6hmde Mammalian expression vector psv23se6hme Mammalian expression vector psv23se6hmk Mammalian expression vector psv23se6hmrk Mammalian expression vector psv51b1sfv Mammalian expression vector psv51e6h Mammalian expression vector psv51e6hf1 Mammalian expression vector psv51e6hf2 Mammalian expression vector psv51e6hmde Mammalian expression vector psv51e6hme Mammalian expression vector psv51e6hmk Mammalian expression vector psv51e6hmrk Marmota monax Megalobrama amblycephala Melanogrammus aeglefinus Meleagris gallopavo Miichthys miiuy Monodelphis domestica Murinae gen. sp. Murine adenovirus 1 Mus Mus musculoides Mus musculus Mus musculus albula Mus musculus castaneus Mus musculus domesticus Mus musculus molossinus Mus musculus musculus Mus musculus, homo sapiens Mus musculus, synthetic construct Mus musculus,homo sapiens Mus pahari Mus sp. Mus spretus Mustela Mustela sp. Myotis lucifugus Myxocyprinus asiaticus Neovison vison Nomascus leucogenys, homo sapiens Notamacropus eugenii Notothenia coriiceps Nyctereutes procyonoides Nycticebus coucang Oncorhynchus Oncorhynchus mykiss Onychogalea fraenata Orectolobus maculatus Orectolobus maculatus, homo sapiens Oreochromis niloticus Ornithorhynchus anatinus Oryctolagus Oryctolagus cuniculus Oryctolagus cuniculus algirus Oryctolagus cuniculus, homo sapiens Oryzias latipes Oves Ovis aries Pagrus major Pan Pan troglodytes Papio anubis Paralichthys olivaceus Pelodiscus sinensis Phagemid vector pcomb3x-kd247scfv-vi Phagemid vector pgald7 Phagemid vector pgald7dl Phagemid vector pgald9 Phagemid vector pgald9dl Phasianidae meleagris Pinnipedia Pisces Plasmid pad1 Plecoglossus altivelis Plecoglossus altivelis altivelis Pleurodeles waltl Polyodontidae Protopterus aethiopicus Protopterus annectens Protopterus dolloi Pseudemys scripta elegans Pseudobatos productus Pteropus alecto Rachycentron canadum Raja eglanteria Raja erinacea Rajiformes dasyatidae Rana catesbeiana Rattus Rattus norvegicus Rattus rattus Rattus sp. Rattus villosissimus Rattus, homo sapiens Red display vector Rpl23-fusion expression vector pscfv Salamandridae Salmo salar Salvelinus alpinus Sarcophilus harrisii Schizothorax prenanti Scophthalmus maximus Scyliorhinus canicula Seriola quinqueradiata Serpentes Silurus meridionalis Siniperca chuatsi Sparus aurata Sphoeroides nephelus Squaliobarbus curriculus Squalus acanthias Stegastes partitus Streptomyces avidinii Struthio camelus Sus Sus scrofa Sylvilagus floridanus Synthetic construct Synthetic plasmid prk122 Synthetic plasmid prk123 Synthetic plasmid puc57-hdr-hel23 Systomus sarana Tachyglossus aculeatus Tachysurus fulvidraco Taeniopygia guttata Takifugu rubripes Testudines Tetraodon nigroviridis Thunnus orientalis Tokudaia muenninki Trachemys scripta elegans Trematomus bernacchii Triakis scyllium Trichechus manatus latirostris Trichosurus vulpecula Tursiops truncatus Tympanuchus cupido Unclassified Unclassified sequences Unidentified Unidentified cloning vector Unknown Vector patce6hy3 Vector pbre6l Vector pdmiphoae6l Vector pqe2c11sfv Vector pqeb1sfv Vector tpmv_p-egfp_hacd20 Vector tpmv_p-egfp_haegfr Vicugna pacos Xenopus Xenopus laevis Xenopus laevis/gilli Xenopus leavis Xenopus tropicalis Yeast expression vector pscudmfe6l2 Yeast expression vector pscudt2mfe6h Yeast expression vector pscudt2mfe6l Region HFR1 CDR-H1 HFR2 CDR-H2 HFR3 CDR-H3 HFR4 Length Distribution for HFR1 (Chothia definition) Region Length Non identical Sequences Relative Frequency 1 4 <1% 2 2881 3% 3 2051 2% 4 919 1% 5 666 <1% 6 387 <1% 7 365 <1% 8 1234 1% 9 2693 3% 10 490 <1% 11 1019 1% 12 523 <1% Region Length Non identical Sequences Relative Frequency 13 644 <1% 14 384 <1% 15 568 <1% 16 1650 2% 17 5524 6% 18 3619 4% 19 608 <1% 20 442 <1% 21 1676 2% 22 1283 1% 23 657 <1% 24 4483 5% Region Length Non identical Sequences Relative Frequency 25 52082 60% 26 244 <1% 27 23 <1% 28 85 <1% 29 4 <1% Total 87208 100%