abYsis

Numbering & Regions

Comparison Organism:

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Region Sequence Details

Regions Definition

Region	Sequence Fragment	Residues	Length
HFR1	QVQLQESGGGSVQAGGSLRLACAAPGYTFS	1 - 30	30
CDR-H1	DYCMG	31 - 35	5
HFR2	WFRQAPGKEREEVA	36 - 49	14
CDR-H2	RISGGKRTYYSDSVRG	50 - 65	16
HFR3	RFTISRDDYKNTVWLQMDSLKPEDTAIYYCAR	66 - 97	32
CDR-H3	GGYTTGVCAGGFND	98 - 111	14
HFR4	WGQGTQVTVSS	112 - 122	11
			122

Region	Definition	Sequence Fragment	Residues	Length
HFR1	Chothia	QVQLQESGGGSVQAGGSLRLACAAP-----	1 - 25	25
	AbM	QVQLQESGGGSVQAGGSLRLACAAP-----	1 - 25	25
	Kabat	QVQLQESGGGSVQAGGSLRLACAAPGYTFS	1 - 30	30
	Contact	QVQLQESGGGSVQAGGSLRLACAAPGYTF-	1 - 29	29
	IMGT	QVQLQESGGGSVQAGGSLRLACAAP-----	1 - 25	25
CDR-H1	Chothia	GYTFSDY---	26 - 32	7
	AbM	GYTFSDYCMG	26 - 35	10
	Kabat	-----DYCMG	31 - 35	5
	Contact	----SDYCMG	30 - 35	6
	IMGT	GYTFSDYC--	26 - 33	8
HFR2	Chothia	CMGWFRQAPGKEREEVARI	33 - 51	19
	AbM	---WFRQAPGKEREEVA--	36 - 49	14
	Kabat	---WFRQAPGKEREEVA--	36 - 49	14
	Contact	---WFRQAPGKERE-----	36 - 46	11
	IMGT	-MGWFRQAPGKEREEVAR-	34 - 50	17
CDR-H2	Chothia	-----SGGKR---------	52 - 56	5
	AbM	---RISGGKRTY-------	50 - 58	9
	Kabat	---RISGGKRTYYSDSVRG	50 - 65	16
	Contact	EVARISGGKRTY-------	47 - 58	12
	IMGT	----ISGGKRT--------	51 - 57	7
HFR3	Chothia	TYYSDSVRGRFTISRDDYKNTVWLQMDSLKPEDTAIYYCAR	57 - 97	41
	AbM	--YSDSVRGRFTISRDDYKNTVWLQMDSLKPEDTAIYYCAR	59 - 97	39
	Kabat	---------RFTISRDDYKNTVWLQMDSLKPEDTAIYYCAR	66 - 97	32
	Contact	--YSDSVRGRFTISRDDYKNTVWLQMDSLKPEDTAIYYC--	59 - 95	37
	IMGT	-YYSDSVRGRFTISRDDYKNTVWLQMDSLKPEDTAIYYC--	58 - 95	38
CDR-H3	Chothia	--GGYTTGVCAGGFND	98 - 111	14
	AbM	--GGYTTGVCAGGFND	98 - 111	14
	Kabat	--GGYTTGVCAGGFND	98 - 111	14
	Contact	ARGGYTTGVCAGGFN-	96 - 110	15
	IMGT	ARGGYTTGVCAGGFND	96 - 111	16
HFR4	Chothia	-WGQGTQVTVSS	112 - 122	11
	AbM	-WGQGTQVTVSS	112 - 122	11
	Kabat	-WGQGTQVTVSS	112 - 122	11
	Contact	DWGQGTQVTVSS	111 - 122	12
	IMGT	-WGQGTQVTVSS	112 - 122	11

Accession & Annotations

Accession	AIF28004.1
Source	abYsis-EMBL-IG KJ732837 Submitted 17-JUL-2014 Updated 17-JUL-2014
Name	anti-sfGFP immunoglobulin heavy chain
Clone	NbsfGFP03
Organism	Camelus dromedarius
Chain Type	Heavy
Pairing	None
Identical Protein Sequences	None
References	Twair A., Al-Okla S., Zarkawi M., Abbady A.Q. 'Characterization of camel nanobodies specific for superfolder GFP fusion proteins' Mol. Biol. Rep. 41(10):6887-6898(2014). Abbady A.Q. Submitted (18-APR-2014) to the INSDC. Molecular Biology and Biotechnology, Atomic Energy Commission, Kafar-Souseh, Damascus, Damascus 6091, Syria

Tools

Sequences

Amino Acid Sequence:

QVQLQESGGGSVQAGGSLRLACAAPGYTFSDYCMGWFRQAPGKEREEVARISGGKRTYYS
DSVRGRFTISRDDYKNTVWLQMDSLKPEDTAIYYCARGGYTTGVCAGGFNDWGQGTQVTV
SS

DNA Sequence:

caggtgcagctgcaggagtctgggggaggctcggtgcaggctggagggtccctgagactc
gcctgtgcagcccctggatacactttcagtgactactgcatgggctggttccgccaggct
ccagggaaggagcgcgaggaggtcgcacgtattagtggtggtaaacgtacatattatagc
gactccgtgaggggccgattcaccatctcccgagacgactacaagaacacggtgtggctg
cagatggacagcctgaaacctgaggacacggccatctattactgtgcgcgaggagggtat
acgactggcgtctgtgcaggggggtttaacgactggggccaggggacccaggtcaccgtc
tcctca

Data Source:

Alignment settings:

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