abYsis

Numbering & Regions

Comparison Organism:

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Region Sequence Details

Regions Definition

Region	Sequence Fragment	Residues	Length
HFR1	EVQLVESGGGLVQAGDSLRLSCTASGRTFS	1 - 30	30
CDR-H1	RAVMG	31 - 35	5
HFR2	WFRQAPGKEREFVA	36 - 49	14
CDR-H2	AISAAPGTAYYAFYADSVRG	50 - 69	20
HFR3	RFSISADSAKNTVYLQMNSLKPEDTAVYYCAA	70 - 101	32
CDR-H3	DLKMQVAAYMNQRSVDY	102 - 118	17
HFR4	WGQGTQVTVSS	119 - 129	11
			129

Region	Definition	Sequence Fragment	Residues	Length
HFR1	Chothia	EVQLVESGGGLVQAGDSLRLSCTAS-----	1 - 25	25
	AbM	EVQLVESGGGLVQAGDSLRLSCTAS-----	1 - 25	25
	Kabat	EVQLVESGGGLVQAGDSLRLSCTASGRTFS	1 - 30	30
	Contact	EVQLVESGGGLVQAGDSLRLSCTASGRTF-	1 - 29	29
	IMGT	EVQLVESGGGLVQAGDSLRLSCTAS-----	1 - 25	25
CDR-H1	Chothia	GRTFSRA---	26 - 32	7
	AbM	GRTFSRAVMG	26 - 35	10
	Kabat	-----RAVMG	31 - 35	5
	Contact	----SRAVMG	30 - 35	6
	IMGT	GRTFSRAV--	26 - 33	8
HFR2	Chothia	VMGWFRQAPGKEREFVAAI	33 - 51	19
	AbM	---WFRQAPGKEREFVA--	36 - 49	14
	Kabat	---WFRQAPGKEREFVA--	36 - 49	14
	Contact	---WFRQAPGKERE-----	36 - 46	11
	IMGT	-MGWFRQAPGKEREFVAA-	34 - 50	17
CDR-H2	Chothia	-----SAAPGTAYY---------	52 - 60	9
	AbM	---AISAAPGTAYYAF-------	50 - 62	13
	Kabat	---AISAAPGTAYYAFYADSVRG	50 - 69	20
	Contact	FVAAISAAPGTAYYAF-------	47 - 62	16
	IMGT	----ISAAPGTAYYA--------	51 - 61	11
HFR3	Chothia	AFYADSVRGRFSISADSAKNTVYLQMNSLKPEDTAVYYCAA	61 - 101	41
	AbM	--YADSVRGRFSISADSAKNTVYLQMNSLKPEDTAVYYCAA	63 - 101	39
	Kabat	---------RFSISADSAKNTVYLQMNSLKPEDTAVYYCAA	70 - 101	32
	Contact	--YADSVRGRFSISADSAKNTVYLQMNSLKPEDTAVYYC--	63 - 99	37
	IMGT	-FYADSVRGRFSISADSAKNTVYLQMNSLKPEDTAVYYC--	62 - 99	38
CDR-H3	Chothia	--DLKMQVAAYMNQRSVDY	102 - 118	17
	AbM	--DLKMQVAAYMNQRSVDY	102 - 118	17
	Kabat	--DLKMQVAAYMNQRSVDY	102 - 118	17
	Contact	AADLKMQVAAYMNQRSVD-	100 - 117	18
	IMGT	AADLKMQVAAYMNQRSVDY	100 - 118	19
HFR4	Chothia	-WGQGTQVTVSS	119 - 129	11
	AbM	-WGQGTQVTVSS	119 - 129	11
	Kabat	-WGQGTQVTVSS	119 - 129	11
	Contact	YWGQGTQVTVSS	118 - 129	12
	IMGT	-WGQGTQVTVSS	119 - 129	11

Accession & Annotations

Accession	AGY46249.1
Source	abYsis-EMBL-IG KF553633 Submitted 30-OCT-2013 Updated 30-OCT-2013
Name	single-chain antibody variable region
Clone	A3
Organism	Synthetic construct
Chain Type	Heavy
Pairing	None
Identical Protein Sequences	None
References	Zabetakis D., Anderson G.P., Bayya N., Goldman E.R. 'Contributions of the complementarity determining regions to the thermal stability of a single-domain antibody' PLoS One 8(10):E77678-E77678(2013). Zabetakis D., Anderson G.P., Bayya N., Goldman E.R. Submitted (19-AUG-2013) to the INSDC. Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, 4555 Overlook Ave, Washington, DC 20375, USA

Tools

Sequences

Amino Acid Sequence:

EVQLVESGGGLVQAGDSLRLSCTASGRTFSRAVMGWFRQAPGKEREFVAAISAAPGTAYY
AFYADSVRGRFSISADSAKNTVYLQMNSLKPEDTAVYYCAADLKMQVAAYMNQRSVDYWG
QGTQVTVSS

DNA Sequence:

gaagttcagctggttgaaagcggtggcggcttggtccaggcgggtgacagcctgcgcctg
agctgtacggcaagcggtcgtactttcagccgtgcagtaatgggctggtttcgccaagca
ccgggtaaagagcgtgagttcgtggctgcgatcagcgctgcgccgggtacggcctattac
gccttctacgcagactccgttcgcggtcgttttagcattagcgcggatagcgcgaaaaac
accgtgtacctgcagatgaatagcctgaagccggaggataccgcggtgtattactgcgct
gcagatctgaagatgcaggttgcggcgtatatgaaccaacgttcggtcgactactggggc
caaggtacccaggttaccgttagcagc

Data Source:

Alignment settings:

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