abYsis

Numbering & Regions

Comparison Organism:

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Region Sequence Details

Regions Definition

Region	Sequence Fragment	Residues	Length
HFR1	EVQLQASGGGLVQGGDSLRLSCAASGRTLG	1 - 30	30
CDR-H1	DYGVA	31 - 35	5
HFR2	WFRQAPGKEREFVS	36 - 49	14
CDR-H2	VISRSTIITDYANSVKG	50 - 66	17
HFR3	RFTISRDNAKNAVYLQMNSLKPEDTAVYYCAV	67 - 98	32
CDR-H3	IANPVYATSRNSDDYGH	99 - 115	17
HFR4	WGQGTQVTVSS	116 - 126	11
			126

Region	Definition	Sequence Fragment	Residues	Length
HFR1	Chothia	EVQLQASGGGLVQGGDSLRLSCAAS-----	1 - 25	25
	AbM	EVQLQASGGGLVQGGDSLRLSCAAS-----	1 - 25	25
	Kabat	EVQLQASGGGLVQGGDSLRLSCAASGRTLG	1 - 30	30
	Contact	EVQLQASGGGLVQGGDSLRLSCAASGRTL-	1 - 29	29
	IMGT	EVQLQASGGGLVQGGDSLRLSCAAS-----	1 - 25	25
CDR-H1	Chothia	GRTLGDY---	26 - 32	7
	AbM	GRTLGDYGVA	26 - 35	10
	Kabat	-----DYGVA	31 - 35	5
	Contact	----GDYGVA	30 - 35	6
	IMGT	GRTLGDYG--	26 - 33	8
HFR2	Chothia	GVAWFRQAPGKEREFVSVI	33 - 51	19
	AbM	---WFRQAPGKEREFVS--	36 - 49	14
	Kabat	---WFRQAPGKEREFVS--	36 - 49	14
	Contact	---WFRQAPGKERE-----	36 - 46	11
	IMGT	-VAWFRQAPGKEREFVSV-	34 - 50	17
CDR-H2	Chothia	-----SRSTII---------	52 - 57	6
	AbM	---VISRSTIITD-------	50 - 59	10
	Kabat	---VISRSTIITDYANSVKG	50 - 66	17
	Contact	FVSVISRSTIITD-------	47 - 59	13
	IMGT	----ISRSTIIT--------	51 - 58	8
HFR3	Chothia	TDYANSVKGRFTISRDNAKNAVYLQMNSLKPEDTAVYYCAV	58 - 98	41
	AbM	--YANSVKGRFTISRDNAKNAVYLQMNSLKPEDTAVYYCAV	60 - 98	39
	Kabat	---------RFTISRDNAKNAVYLQMNSLKPEDTAVYYCAV	67 - 98	32
	Contact	--YANSVKGRFTISRDNAKNAVYLQMNSLKPEDTAVYYC--	60 - 96	37
	IMGT	-DYANSVKGRFTISRDNAKNAVYLQMNSLKPEDTAVYYC--	59 - 96	38
CDR-H3	Chothia	--IANPVYATSRNSDDYGH	99 - 115	17
	AbM	--IANPVYATSRNSDDYGH	99 - 115	17
	Kabat	--IANPVYATSRNSDDYGH	99 - 115	17
	Contact	AVIANPVYATSRNSDDYG-	97 - 114	18
	IMGT	AVIANPVYATSRNSDDYGH	97 - 115	19
HFR4	Chothia	-WGQGTQVTVSS	116 - 126	11
	AbM	-WGQGTQVTVSS	116 - 126	11
	Kabat	-WGQGTQVTVSS	116 - 126	11
	Contact	HWGQGTQVTVSS	115 - 126	12
	IMGT	-WGQGTQVTVSS	116 - 126	11

Accession & Annotations

Accession	AGY46250.1
Source	abYsis-EMBL-IG KF553634 Submitted 30-OCT-2013 Updated 30-OCT-2013
Name	single-chain antibody variable region
Clone	C8
Organism	Synthetic construct
Chain Type	Heavy
Pairing	None
Identical Protein Sequences	None
References	Zabetakis D., Anderson G.P., Bayya N., Goldman E.R. 'Contributions of the complementarity determining regions to the thermal stability of a single-domain antibody' PLoS One 8(10):E77678-E77678(2013). Zabetakis D., Anderson G.P., Bayya N., Goldman E.R. Submitted (19-AUG-2013) to the INSDC. Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, 4555 Overlook Ave, Washington, DC 20375, USA

Tools

Sequences

Amino Acid Sequence:

EVQLQASGGGLVQGGDSLRLSCAASGRTLGDYGVAWFRQAPGKEREFVSVISRSTIITDY
ANSVKGRFTISRDNAKNAVYLQMNSLKPEDTAVYYCAVIANPVYATSRNSDDYGHWGQGT
QVTVSS

DNA Sequence:

gaggtgcagctgcaggcgtctgggggaggattggtgcagggtggggactctctgagactc
tcctgtgcagcctctggacgcaccctcggcgactatggcgtggcctggttccgccaggct
ccagggaaggagcgtgagtttgtatcagttattagtcgtagtacaattatcacagactat
gcaaactccgtgaagggccgattcaccatctccagagacaacgccaagaatgcggtgtat
ctgcaaatgaacagcctgaaacctgaggacacggccgtttattattgcgcagtgatagca
aatccagtctatgctactagtagaaactcggatgactatggtcactggggccaggggacc
caggtcaccgtctcctca

Data Source:

Alignment settings:

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