abYsis

Numbering & Regions

Comparison Organism:

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Region Sequence Details

Regions Definition

Region	Sequence Fragment	Residues	Length
HFR1	QVQLQESGGGLVQAGGSLRLSCTASGRTFS	1 - 30	30
CDR-H1	SYAMG	31 - 35	5
HFR2	WFRQTPGKEREFVA	36 - 49	14
CDR-H2	AITWGGSTTLYADSVKG	50 - 66	17
HFR3	RFTMSRDNAKNTVYLQMNSLKPEDTAVYYCAA	67 - 98	32
CDR-H3	DGSQYRSTYSFRDKPDYGS	99 - 117	19
HFR4	WGQGTQVTVSS	118 - 128	11
Tail	HHHHHH	128 - 134	6
			134

Region	Definition	Sequence Fragment	Residues	Length
HFR1	Chothia	QVQLQESGGGLVQAGGSLRLSCTAS-----	1 - 25	25
	AbM	QVQLQESGGGLVQAGGSLRLSCTAS-----	1 - 25	25
	Kabat	QVQLQESGGGLVQAGGSLRLSCTASGRTFS	1 - 30	30
	Contact	QVQLQESGGGLVQAGGSLRLSCTASGRTF-	1 - 29	29
	IMGT	QVQLQESGGGLVQAGGSLRLSCTAS-----	1 - 25	25
CDR-H1	Chothia	GRTFSSY---	26 - 32	7
	AbM	GRTFSSYAMG	26 - 35	10
	Kabat	-----SYAMG	31 - 35	5
	Contact	----SSYAMG	30 - 35	6
	IMGT	GRTFSSYA--	26 - 33	8
HFR2	Chothia	AMGWFRQTPGKEREFVAAI	33 - 51	19
	AbM	---WFRQTPGKEREFVA--	36 - 49	14
	Kabat	---WFRQTPGKEREFVA--	36 - 49	14
	Contact	---WFRQTPGKERE-----	36 - 46	11
	IMGT	-MGWFRQTPGKEREFVAA-	34 - 50	17
CDR-H2	Chothia	-----TWGGST---------	52 - 57	6
	AbM	---AITWGGSTTL-------	50 - 59	10
	Kabat	---AITWGGSTTLYADSVKG	50 - 66	17
	Contact	FVAAITWGGSTTL-------	47 - 59	13
	IMGT	----ITWGGSTT--------	51 - 58	8
HFR3	Chothia	TLYADSVKGRFTMSRDNAKNTVYLQMNSLKPEDTAVYYCAA	58 - 98	41
	AbM	--YADSVKGRFTMSRDNAKNTVYLQMNSLKPEDTAVYYCAA	60 - 98	39
	Kabat	---------RFTMSRDNAKNTVYLQMNSLKPEDTAVYYCAA	67 - 98	32
	Contact	--YADSVKGRFTMSRDNAKNTVYLQMNSLKPEDTAVYYC--	60 - 96	37
	IMGT	-LYADSVKGRFTMSRDNAKNTVYLQMNSLKPEDTAVYYC--	59 - 96	38
CDR-H3	Chothia	--DGSQYRSTYSFRDKPDYGS	99 - 117	19
	AbM	--DGSQYRSTYSFRDKPDYGS	99 - 117	19
	Kabat	--DGSQYRSTYSFRDKPDYGS	99 - 117	19
	Contact	AADGSQYRSTYSFRDKPDYG-	97 - 116	20
	IMGT	AADGSQYRSTYSFRDKPDYGS	97 - 117	21
HFR4	Chothia	-WGQGTQVTVSS	118 - 128	11
	AbM	-WGQGTQVTVSS	118 - 128	11
	Kabat	-WGQGTQVTVSS	118 - 128	11
	Contact	SWGQGTQVTVSS	117 - 128	12
	IMGT	-WGQGTQVTVSS	118 - 128	11

Accession & Annotations

Accession	4ej1_C
Source	PDB 4ej1 Submitted 06-APR-2012 Updated 30-OCT-2013
Name	BINDING OF NB113 CAMELID ANTIBODY FRAGMENT WITH THE BINARY DHFR:FOLATE COMPLEX
Organism	Lama glama
Chain Type	Heavy
Pairing	None
Identical Protein Sequences	PDB: 4eiz_C 4eiz_D 4ej1_D
References	OYEN D., WECHSELBERGER R., SRINIVASAN V., STEYAERT J., BARLOW N. 'MECHANISTIC ANALYSIS OF ALLOSTERIC AND NON-ALLOSTERIC EFFECTS ARISING FROM NANOBODY BINDING TO TWO EPITOPES OF THE DIHYROFOLATE REDUCTASE OF ESCHERICHIA COLI.' BIOCHIM.BIOPHYS.ACTA V.1834 2147 2013 ISSN 0006-3002

Tools

Sequences

Amino Acid Sequence:

QVQLQESGGGLVQAGGSLRLSCTASGRTFSSYAMGWFRQTPGKEREFVAAITWGGSTTLY
ADSVKGRFTMSRDNAKNTVYLQMNSLKPEDTAVYYCAADGSQYRSTYSFRDKPDYGSWGQ
GTQVTVSSHHHHHH