abYsis

Numbering & Regions

Comparison Organism:

Calculating, please wait ...

Region Sequence Details

Regions Definition

Region	Sequence Fragment	Residues	Length
HFR1	QVQLQESGGGLVQAGGSLRLSCEASGRTFS	1 - 30	30
CDR-H1	SYAMG	31 - 35	5
HFR2	WFRQAPGKERDFVA	36 - 49	14
CDR-H2	VISWSGSNTYYADSAKG	50 - 66	17
HFR3	RFTISRDNAKNTVYLQMNSLKPEDTAIYYCAA	67 - 98	32
CDR-H3	PGRPHGSSWSLNKKGQGYDY	99 - 118	20
HFR4	WGQGTQVTVSS	119 - 129	11
Tail	HHHHHH	129 - 135	6
			135

Region	Definition	Sequence Fragment	Residues	Length
HFR1	Chothia	QVQLQESGGGLVQAGGSLRLSCEAS-----	1 - 25	25
	AbM	QVQLQESGGGLVQAGGSLRLSCEAS-----	1 - 25	25
	Kabat	QVQLQESGGGLVQAGGSLRLSCEASGRTFS	1 - 30	30
	Contact	QVQLQESGGGLVQAGGSLRLSCEASGRTF-	1 - 29	29
	IMGT	QVQLQESGGGLVQAGGSLRLSCEAS-----	1 - 25	25
CDR-H1	Chothia	GRTFSSY---	26 - 32	7
	AbM	GRTFSSYAMG	26 - 35	10
	Kabat	-----SYAMG	31 - 35	5
	Contact	----SSYAMG	30 - 35	6
	IMGT	GRTFSSYA--	26 - 33	8
HFR2	Chothia	AMGWFRQAPGKERDFVAVI	33 - 51	19
	AbM	---WFRQAPGKERDFVA--	36 - 49	14
	Kabat	---WFRQAPGKERDFVA--	36 - 49	14
	Contact	---WFRQAPGKERD-----	36 - 46	11
	IMGT	-MGWFRQAPGKERDFVAV-	34 - 50	17
CDR-H2	Chothia	-----SWSGSN---------	52 - 57	6
	AbM	---VISWSGSNTY-------	50 - 59	10
	Kabat	---VISWSGSNTYYADSAKG	50 - 66	17
	Contact	FVAVISWSGSNTY-------	47 - 59	13
	IMGT	----ISWSGSNT--------	51 - 58	8
HFR3	Chothia	TYYADSAKGRFTISRDNAKNTVYLQMNSLKPEDTAIYYCAA	58 - 98	41
	AbM	--YADSAKGRFTISRDNAKNTVYLQMNSLKPEDTAIYYCAA	60 - 98	39
	Kabat	---------RFTISRDNAKNTVYLQMNSLKPEDTAIYYCAA	67 - 98	32
	Contact	--YADSAKGRFTISRDNAKNTVYLQMNSLKPEDTAIYYC--	60 - 96	37
	IMGT	-YYADSAKGRFTISRDNAKNTVYLQMNSLKPEDTAIYYC--	59 - 96	38
CDR-H3	Chothia	--PGRPHGSSWSLNKKGQGYDY	99 - 118	20
	AbM	--PGRPHGSSWSLNKKGQGYDY	99 - 118	20
	Kabat	--PGRPHGSSWSLNKKGQGYDY	99 - 118	20
	Contact	AAPGRPHGSSWSLNKKGQGYD-	97 - 117	21
	IMGT	AAPGRPHGSSWSLNKKGQGYDY	97 - 118	22
HFR4	Chothia	-WGQGTQVTVSS	119 - 129	11
	AbM	-WGQGTQVTVSS	119 - 129	11
	Kabat	-WGQGTQVTVSS	119 - 129	11
	Contact	YWGQGTQVTVSS	118 - 129	12
	IMGT	-WGQGTQVTVSS	119 - 129	11

Accession & Annotations

Accession	4fhb_D
Source	PDB 4fhb Submitted 06-JUN-2012 Updated 15-NOV-2017
Name	ENHANCING DHFR CATALYSIS BY BINDING OF AN ALLOSTERIC REGULATOR NANOBODY (NB179)
Organism	Lama glama
Chain Type	Heavy
Pairing	None
Identical Protein Sequences	None
References	OYEN D., WECHSELBERGER R., SRINIVASAN V., STEYAERT J., BARLOW N. 'MECHANISTIC ANALYSIS OF ALLOSTERIC AND NON-ALLOSTERIC EFFECTS ARISING FROM NANOBODY BINDING TO TWO EPITOPES OF THE DIHYROFOLATE REDUCTASE OF ESCHERICHIA COLI.' BIOCHIM.BIOPHYS.ACTA V.1834 2147 2013 ISSN 0006-3002

Tools

Sequences

Amino Acid Sequence:

QVQLQESGGGLVQAGGSLRLSCEASGRTFSSYAMGWFRQAPGKERDFVAVISWSGSNTYY
ADSAKGRFTISRDNAKNTVYLQMNSLKPEDTAIYYCAAPGRPHGSSWSLNKKGQGYDYWG
QGTQVTVSSHHHHHH