abYsis

Numbering & Regions

Comparison Organism:

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Region Sequence Details

Regions Definition

Region	Sequence Fragment	Residues	Length
LFR1	DIELTQSPAIMAASPGEKITITC	1 - 23	23
CDR-L1	SASSSISSNYLH	24 - 35	12
LFR2	WYQQKPGFSPKLLIY	36 - 50	15
CDR-L2	RTSNLAS	51 - 57	7
LFR3	GVPARFSGSGSGTSYSLTISRMEAEDAATYYC	58 - 89	32
CDR-L3	QQRSSYPLT	90 - 98	9
LFR4	FGAGTKLELKRAA	99 - 111	13
Tail	ALE	111 - 114	3
			114

Region	Definition	Sequence Fragment	Residues	Length
LFR1	Chothia	DIELTQSPAIMAASPGEKITITC------	1 - 23	23
	AbM	DIELTQSPAIMAASPGEKITITC------	1 - 23	23
	Kabat	DIELTQSPAIMAASPGEKITITC------	1 - 23	23
	Contact	DIELTQSPAIMAASPGEKITITCSASSSI	1 - 29	29
	IMGT	DIELTQSPAIMAASPGEKITITCSAS---	1 - 26	26
CDR-L1	Chothia	SASSSISSNYLH--	24 - 35	12
	AbM	SASSSISSNYLH--	24 - 35	12
	Kabat	SASSSISSNYLH--	24 - 35	12
	Contact	------SSNYLHWY	30 - 37	8
	IMGT	---SSISSNY----	27 - 33	7
LFR2	Chothia	--WYQQKPGFSPKLLIY	36 - 50	15
	AbM	--WYQQKPGFSPKLLIY	36 - 50	15
	Kabat	--WYQQKPGFSPKLLIY	36 - 50	15
	Contact	----QQKPGFSPK----	38 - 46	9
	IMGT	LHWYQQKPGFSPKLLIY	34 - 50	17
CDR-L2	Chothia	----RTSNLAS	51 - 57	7
	AbM	----RTSNLAS	51 - 57	7
	Kabat	----RTSNLAS	51 - 57	7
	Contact	LLIYRTSNLA-	47 - 56	10
	IMGT	----RT-----	51 - 52	2
LFR3	Chothia	-----GVPARFSGSGSGTSYSLTISRMEAEDAATYYC	58 - 89	32
	AbM	-----GVPARFSGSGSGTSYSLTISRMEAEDAATYYC	58 - 89	32
	Kabat	-----GVPARFSGSGSGTSYSLTISRMEAEDAATYYC	58 - 89	32
	Contact	----SGVPARFSGSGSGTSYSLTISRMEAEDAATYYC	57 - 89	33
	IMGT	SNLASGVPARFSGSGSGTSYSLTISRMEAEDAATYYC	53 - 89	37
CDR-L3	Chothia	QQRSSYPLT	90 - 98	9
	AbM	QQRSSYPLT	90 - 98	9
	Kabat	QQRSSYPLT	90 - 98	9
	Contact	QQRSSYPL-	90 - 97	8
	IMGT	QQRSSYPLT	90 - 98	9
LFR4	Chothia	-FGAGTKLELKRAA	99 - 111	13
	AbM	-FGAGTKLELKRAA	99 - 111	13
	Kabat	-FGAGTKLELKRAA	99 - 111	13
	Contact	TFGAGTKLELKRAA	98 - 111	14
	IMGT	-FGAGTKLELKRAA	99 - 111	13

Accession & Annotations

Accession	ABC94917.1
Source	abYsis-EMBL-IG DQ358915 Submitted 07-FEB-2006 Updated 22-AUG-2011
Name	anti-rhGM-CSF light chain variable domain
Clone	196
Organism	Synthetic construct
Chain Type	Light
Pairing	ABC94918.1
Identical Protein Sequences	None
References	Tapryal S., Mukherjee K.J. Submitted (11-JAN-2006) to the INSDC. Centre for Biotechnology, Jawaharlal Nehru University, New Delhi, Delhi 110067, India Tapryal S., Pal Khasa Y., Mukherjee K.J. 'Single chain Fv fragment specific for human GM-CSF: selection and expression using a bacterial expression library' Biotechnol J 5(10):1078-1089(2010). Tapryal S., Mukherjee K.J. 'Cloning and expression of single chain antibody (scFv) to rhGM-CSF in E. coli' Unpublished.

Tools

Sequences

Amino Acid Sequence:

DIELTQSPAIMAASPGEKITITCSASSSISSNYLHWYQQKPGFSPKLLIYRTSNLASGVP
ARFSGSGSGTSYSLTISRMEAEDAATYYCQQRSSYPLTFGAGTKLELKRAAALE

DNA Sequence:

gacattgagctcacccagtctccagcaatcatggctgcatctcccggggagaagatcact
atcacctgcagtgccagctcaagtataagttccaattacttgcattggtatcagcagaag
ccaggattctcccctaaactcttgatttataggacatccaatctggcttctggagtccca
gctcgcttcagtggcagtgggtctgggacctcttactctctcacaatcagccgaatggag
gctgaagatgctgccacttattactgccagcaaaggagtagttacccactcacgttcggt
gctgggaccaagctggagctgaaacgggcggccgcactcgag

Data Source:

Alignment settings:

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