abYsis

Numbering & Regions

Comparison Organism:

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Region Sequence Details

Regions Definition

Region	Sequence Fragment	Residues	Length
HFR1	QVKLQQSGAELVRPGASVKLSCKASGYTFT	1 - 30	30
CDR-H1	SYWMN	31 - 35	5
HFR2	WVKQRPEQGLEWIG	36 - 49	14
CDR-H2	RIDPYDSETHYNQKFKD	50 - 66	17
HFR3	KAILTVDKSSSTAYMQLSSLTSEDSAVYFCAP	67 - 98	32
CDR-H3	YYYGSSYWYFDV	99 - 110	12
HFR4	WGQGTTVTVS	111 - 120	10
			120

Region	Definition	Sequence Fragment	Residues	Length
HFR1	Chothia	QVKLQQSGAELVRPGASVKLSCKAS-----	1 - 25	25
	AbM	QVKLQQSGAELVRPGASVKLSCKAS-----	1 - 25	25
	Kabat	QVKLQQSGAELVRPGASVKLSCKASGYTFT	1 - 30	30
	Contact	QVKLQQSGAELVRPGASVKLSCKASGYTF-	1 - 29	29
	IMGT	QVKLQQSGAELVRPGASVKLSCKAS-----	1 - 25	25
CDR-H1	Chothia	GYTFTSY---	26 - 32	7
	AbM	GYTFTSYWMN	26 - 35	10
	Kabat	-----SYWMN	31 - 35	5
	Contact	----TSYWMN	30 - 35	6
	IMGT	GYTFTSYW--	26 - 33	8
HFR2	Chothia	WMNWVKQRPEQGLEWIGRI	33 - 51	19
	AbM	---WVKQRPEQGLEWIG--	36 - 49	14
	Kabat	---WVKQRPEQGLEWIG--	36 - 49	14
	Contact	---WVKQRPEQGLE-----	36 - 46	11
	IMGT	-MNWVKQRPEQGLEWIGR-	34 - 50	17
CDR-H2	Chothia	-----DPYDSE---------	52 - 57	6
	AbM	---RIDPYDSETH-------	50 - 59	10
	Kabat	---RIDPYDSETHYNQKFKD	50 - 66	17
	Contact	WIGRIDPYDSETH-------	47 - 59	13
	IMGT	----IDPYDSET--------	51 - 58	8
HFR3	Chothia	THYNQKFKDKAILTVDKSSSTAYMQLSSLTSEDSAVYFCAP	58 - 98	41
	AbM	--YNQKFKDKAILTVDKSSSTAYMQLSSLTSEDSAVYFCAP	60 - 98	39
	Kabat	---------KAILTVDKSSSTAYMQLSSLTSEDSAVYFCAP	67 - 98	32
	Contact	--YNQKFKDKAILTVDKSSSTAYMQLSSLTSEDSAVYFC--	60 - 96	37
	IMGT	-HYNQKFKDKAILTVDKSSSTAYMQLSSLTSEDSAVYFC--	59 - 96	38
CDR-H3	Chothia	--YYYGSSYWYFDV	99 - 110	12
	AbM	--YYYGSSYWYFDV	99 - 110	12
	Kabat	--YYYGSSYWYFDV	99 - 110	12
	Contact	APYYYGSSYWYFD-	97 - 109	13
	IMGT	APYYYGSSYWYFDV	97 - 110	14
HFR4	Chothia	-WGQGTTVTVS	111 - 120	10
	AbM	-WGQGTTVTVS	111 - 120	10
	Kabat	-WGQGTTVTVS	111 - 120	10
	Contact	VWGQGTTVTVS	110 - 120	11
	IMGT	-WGQGTTVTVS	111 - 120	10

Accession & Annotations

Accession	ABC94918.1
Source	abYsis-EMBL-IG DQ358916 Submitted 07-FEB-2006 Updated 22-AUG-2011
Name	anti-rhGM-CSF heavy chain variable domain
Clone	196
Organism	Synthetic construct
Chain Type	Heavy
Pairing	ABC94917.1
Identical Protein Sequences	None
References	Tapryal S., Mukherjee K.J. Submitted (11-JAN-2006) to the INSDC. Centre for Biotechnology, Jawaharlal Nehru University, New Delhi, Delhi 110067, India Tapryal S., Pal Khasa Y., Mukherjee K.J. 'Single chain Fv fragment specific for human GM-CSF: selection and expression using a bacterial expression library' Biotechnol J 5(10):1078-1089(2010). Tapryal S., Mukherjee K.J. 'Cloning and expression of single chain antibody (scFv) to rhGM-CSF in E. coli' Unpublished.

Tools

Sequences

Amino Acid Sequence:

QVKLQQSGAELVRPGASVKLSCKASGYTFTSYWMNWVKQRPEQGLEWIGRIDPYDSETHY
NQKFKDKAILTVDKSSSTAYMQLSSLTSEDSAVYFCAPYYYGSSYWYFDVWGQGTTVTVS

DNA Sequence:

caggtcaaactgcagcagtcaggggctgagctggtgaggcctggggcttcagtgaagctg
tcctgcaaggcttctggctacacgttcaccagctactggatgaactgggttaagcagagg
cctgagcaaggccttgagtggattggaaggattgatccttacgatagtgaaactcactac
aatcaaaagttcaaggacaaggccatattgactgtagacaaatcctccagcacagcctac
atgcaactcagcagcctgacatctgaggactctgcggtctatttctgtgcgccttattac
tacggtagtagctactggtacttcgatgtctggggccaagggaccacggtcaccgtctcc

Data Source:

Alignment settings:

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